In a study of the molecular¹ underpinnings of plants' pest resistance, Rice University biologists have shown that plants both anticipate daytime raids by hungry insects and make sophisticated preparations to fend² them off. "When you walk past plants, they don't look like they're doing anything," said Janet Braam, an investigator³ on the new study, which appears this week in the Proceedings⁴ of the National Academy of Sciences. "It's intriguing⁵（有趣的） to see all of this activity down at the genetic⁶ level. It's like watching a besieged⁷（包围，围绕） fortress⁸ go on full alert."

Braam, professor and chair of Rice's Department of Biochemistry and Cell Biology, said scientists have long known that plants have an internal clock that allows them to measure time regardless of light conditions. For example, some plants that track the sun with their leaves during the day are known to "reset⁹" their leaves at night and move them back toward the east in anticipation¹⁰ of sunrise.

In recent years, scientists have begun to apply powerful genetic tools to the study of plant circadian（生理节律的） rhythms. Researchers have found that as many as one-third of the genes¹¹ in Arabidopsis thaliana -- a widely studied species in plant biology -- are activated¹² by the circadian cycle. Rice biochemist Michael Covington found that some of these circadian-regulated genes were also connected to wounding responses.

"We wondered whether some of these circadian-regulated genes might allow plants to anticipate attacks from insects, in much the same way that they anticipate the sunrise," said Covington, now at the University of California, Davis.

Danielle Goodspeed, a graduate student in biochemistry and cell biology, designed a clever experiment to answer the question. She used 12-hour light cycles to entrain the circadian clocks of both Arabidopsis plants and cabbage loopers, a type of caterpillar¹³ that eats Arabidopsis. Half of the plants were placed with caterpillars¹⁴ on a regular day-night cycle, and the other half were placed with "out-of-phase" caterpillars whose internal clocks were set to daytime mode during the hours that the plants were in nighttime mode.