文章来源:未知 文章作者:meng 发布时间:2010-08-04 01:29 字体: [ ]  进入论坛

Scientists hoping to understand how cells work may get a boost from a new technique to tag and image proteins within living mammalian(哺乳类) cells. The new technique, developed by a research team led by University of Illinois at Chicago assistant professor of chemistry Lawrence Miller1, provides the clearest, most dynamic(动态的) view yet of protein-protein interactions in cells when viewed through a specially2 modified microscope.

The finding is reported in the Proceedings3 of the National Academy of Sciences (advanced online July 19.)

Knowing where and when particular proteins interact within the cell is key to understanding life processes at the molecular4 level.

In a technique called luminescence(发冷光) resonance5 energy transfer, two proteins in a cell are labeled with differently colored, luminescent molecules6 that absorb light of one color and give it off as another color. By taking several pictures of the cell and mathematically analyzing8 the pictures, researchers gain information about the proteins' precise location and whether they are interacting.

Miller and his team used a novel type of luminescent molecule7 for labeling, making it possible to get the same information using fewer pictures. This simplifies the analysis and allows for five-fold faster data acquisition(数据采集) . Images show a 50-fold improvement in sensitivity.

Working with Jerrold Turner, professor and associate head of pathology(病理学) at the University of Chicago, Miller used a hybrid9 chemical/genetic approach to tag the proteins of interest. One of the proteins was genetically10 modified so that it would bind11 to a terbium complex. The terbium complex has an unusually long time between light absorption and emission12. The second target protein was genetically modified to link to a fluorescent13 tag with a short emission lifetime. When the two proteins interact, the luminescent tags are brought very close together, generating a unique luminescent signal that can be seen under a microscope.

Miller and his colleagues modified a conventional microscope to exploit the long lifetime of the terbium protein tags. Pulsed light is used to trigger the terbium luminescence, detected after the other luminescent species within cells have gone dark, allowing unwanted background to be removed from the image.

The new technique "increases sensitivity and makes the whole process faster," Miller said. "This increases the time-resolution of the experiment, allowing you to see how interactions change on a faster time scale, which can help to better figure out how certain biological phenomena14 work."

The technique required a reliable(可靠的) way to deliver the luminescent terbium probe through a living cell membrane15 without contaminating or damaging the cell. The researchers developed a way to co-opt pinocytosis(饮液) , the process by which cells drink in small amounts of surrounding fluid.

"With this new tool, we hope cell biologists and others will be able to study things they haven't seen before, such as interactions that couldn't be visualized16 in live cells in real time," Miller said. "Hopefully the method will yield information that makes it easier to deduce biological mechanisms17."


1 miller ZD6xf     
  • Every miller draws water to his own mill.磨坊主都往自己磨里注水。
  • The skilful miller killed millions of lions with his ski.技术娴熟的磨坊主用雪橇杀死了上百万头狮子。
2 specially Hviwq     
  • They are specially packaged so that they stack easily.它们经过特别包装以便于堆放。
  • The machine was designed specially for demolishing old buildings.这种机器是专为拆毁旧楼房而设计的。
3 proceedings Wk2zvX     
  • He was released on bail pending committal proceedings. 他交保获释正在候审。
  • to initiate legal proceedings against sb 对某人提起诉讼
4 molecular mE9xh     
  • The research will provide direct insight into molecular mechanisms.这项研究将使人能够直接地了解分子的机理。
  • For the pressure to become zero, molecular bombardment must cease.当压强趋近于零时,分子的碰撞就停止了。
5 resonance hBazC     
  • Playing the piano sets up resonance in those glass ornaments.一弹钢琴那些玻璃饰物就会产生共振。
  • The areas under the two resonance envelopes are unequal.两个共振峰下面的面积是不相等的。
6 molecules 187c25e49d45ad10b2f266c1fa7a8d49     
分子( molecule的名词复数 )
  • The structure of molecules can be seen under an electron microscope. 分子的结构可在电子显微镜下观察到。
  • Inside the reactor the large molecules are cracked into smaller molecules. 在反应堆里,大分子裂变为小分子。
7 molecule Y6Tzn     
  • A molecule of water is made up of two atoms of hygrogen and one atom of oxygen.一个水分子是由P妈?f婘??妈?成的。
  • This gives us the structural formula of the molecule.这种方式给出了分子的结构式。
8 analyzing be408cc8d92ec310bb6260bc127c162b     
v.分析;分析( analyze的现在分词 );分解;解释;对…进行心理分析n.分析
  • Analyzing the date of some socialist countries presents even greater problem s. 分析某些社会主义国家的统计数据,暴露出的问题甚至更大。 来自辞典例句
  • He undoubtedly was not far off the mark in analyzing its predictions. 当然,他对其预测所作的分析倒也八九不离十。 来自辞典例句
9 hybrid pcBzu     
  • That is a hybrid perpetual rose.那是一株杂交的四季开花的蔷薇。
  • The hybrid was tall,handsome,and intelligent.那混血儿高大、英俊、又聪明。
10 genetically Lgixo     
  • All the bees in the colony are genetically related. 同一群体的蜜蜂都有亲缘关系。
  • Genetically modified foods have already arrived on American dinner tables. 经基因改造加工过的食物已端上了美国人的餐桌。 来自英汉非文学 - 生命科学 - 基因与食物
11 bind Vt8zi     
  • I will let the waiter bind up the parcel for you.我让服务生帮你把包裹包起来。
  • He wants a shirt that does not bind him.他要一件不使他觉得过紧的衬衫。
12 emission vjnz4     
  • Rigorous measures will be taken to reduce the total pollutant emission.采取严格有力措施,降低污染物排放总量。
  • Finally,the way to effectively control particulate emission is pointed out.最后,指出有效降低颗粒排放的方向。
13 fluorescent Zz2y3     
  • They observed the deflections of the particles by allowing them to fall on a fluorescent screen.他们让粒子落在荧光屏上以观察他们的偏移。
  • This fluorescent lighting certainly gives the food a peculiar color.这萤光灯当然增添了食物特别的色彩。
14 phenomena 8N9xp     
  • Ade couldn't relate the phenomena with any theory he knew.艾德无法用他所知道的任何理论来解释这种现象。
  • The object of these experiments was to find the connection,if any,between the two phenomena.这些实验的目的就是探索这两种现象之间的联系,如果存在着任何联系的话。
15 membrane H7ez8     
  • A vibrating membrane in the ear helps to convey sounds to the brain.耳膜的振动帮助声音传送到大脑。
  • A plastic membrane serves as selective diffusion barrier.一层塑料薄膜起着选择性渗透屏障的作用。
16 visualized 052bbebb5da308bd361d83e229771079     
  • I had visualized scientists as bearded old men. 我曾经把科学家想像成长满胡子的老人。
  • "I visualized mangled and inadequate branches for my fires. 我想像中出现了砍得乱七八糟的树枝子,供不上壁炉烧的。 来自名作英译部分
17 mechanisms d0db71d70348ef1c49f05f59097917b8     
n.机械( mechanism的名词复数 );机械装置;[生物学] 机制;机械作用
  • The research will provide direct insight into molecular mechanisms. 这项研究将使人能够直接地了解分子的机理。 来自《简明英汉词典》
  • He explained how the two mechanisms worked. 他解释这两台机械装置是如何工作的。 来自《简明英汉词典》