Numerous studies have linked exposure to bisphenol A (BPA) in plastic, receipt paper, toys, and other products with various health problems from poor growth to cancer, and the FDA has been supporting efforts to find and use alternatives. But are these alternatives safer? Researchers reporting in the Cell Press journal Chemistry & Biology have developed new tests that can classify such compounds' activity with great detail and speed. The advance could offer a fast and cost-effective way to identify safe replacements¹ for BPA. Millions of tons of BPA and related compounds are produced each year. "I think it is fair to say that many of these BPA analogs（类似物） have not been thoroughly² tested, yet they are used in everyday plastics such as water bottles, baby bottles, and the lining³ of canned goods." says lead author Dr. Fabio Stossi of Baylor College of Medicine.

 

BPA and BPA analogs belong to a class of compounds called endocrine（内分泌，激素） disruptors, so named because they can interfere⁴ with the body's endocrine, or hormonal⁵, system. Using their newly developed assays⁶ on living cells, Dr. Stossi and his colleagues characterized how 18 different BPA analogs affect alpha and beta estrogen receptors, which are the primary targets of this class of chemicals. Their studies were conducted using high throughput microscopy and automated⁷ image analysis in different cell line models, with varying exposures to BPA analogs.

 

The investigators⁸ were able to record and analyze⁹ massive data sets related to BPA analogs. "The high throughput approach that we've refined during the past several years can simultaneously¹⁰ quantify what these compounds are doing to a wide range of processes such as protein levels, nuclear trafficking, DNA¹¹ binding¹², protein interactions, transcription, cell cycle, and proliferation," says senior author Dr. Michael A. Mancini, of Baylor and the Texas A&M Health Science Center Institute of Biosciences and Technology (IBT). "The results showed us that various BPA analogs increased or decreased certain receptor activities, while others were receptor specific; clearly, the various BPA analogs can have unique properties."

 

The investigators found that many BPA analogs have inhibitory effects on the beta form of the estrogen（雌性激素） receptor, a less well-studied steroid receptor that has tumor¹³ fighting properties. Many analogs also acted to stimulate¹⁴ the alpha form of the estrogen receptor or they had mixed inhibitory and stimulatory¹⁵ effects. Determining precisely¹⁶ how these effects influence human health will require additional research. "These studies represent a breakthrough in our ability to focus precious resources on those BPA analogs and other endocrine disrupting chemicals of greatest concern," says coauthor Dr. Cheryl Walker of the IBT.

 

The scientists note that there are likely many more BPA-like compounds that can be found in products and in the environment. The widely applicable technologies used in the study will enable investigators to rapidly test such compounds for any unexpected or undesirable¹⁷ properties.

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