Researchers from the Georgia Institute of Technology and the Centers for Disease Control and Prevention (CDC) have developed a new laboratory test that can rapidly identify the bacterium1(细菌) responsible for staph(葡萄球菌) infections. This new test takes advantage of unique isotopic2 labeling combined with specific bacteriophage(噬菌体) amplification3 to rapidly identify Staphylococcus aureus. Quickly and accurately4 detecting infections caused by S. aureus is critical because the pathogenic bacterium causes a broad spectrum5 of infections, ranging from acute to chronic6 disease, which need to be treated in a prompt manner with the correct antibiotic7.
The test uses mass spectrometry to quantify the number of S. aureus organisms in a large number of samples in just a few hours, compared to a day or two for culturing techniques typically used to detect this bacterium.
"Our method for detecting staph infections using mass spectrometry will be valuable in a variety of situations, but will be crucial when a large number of people need to be tested very quickly, which will ultimately improve treatment," said Facundo Fernández, an associate professor in the Georgia Tech School of Chemistry and Biochemistry.
Details of the new staph infection detection method were published in the January issue of the journal Molecular8 and Cellular9 Proteomics. Partial funding for this research was provided by 3M and the CDC/Georgia Tech seed award program.
Fernández together with Carrie Pierce, Jon Rees and John Barr from the CDC's Division of Laboratory Sciences created this test.
"The simplicity10 of sample preparation, the low cost of required reagents(试剂) and the increased availability of mass spectrometers in clinical laboratories make this new method a cost-effective way to rapidly and effectively detect staph infections, which must be treated quickly to prevent spread of the disease," explained Pierce, a research chemist at the CDC who also worked on the project as a graduate student at Georgia Tech.
To run their test, the researchers first inject a known amount of bacteriophage labeled with nitrogen-15 into a sample. The phages -- which are viruses that infect bacteria -- infect only live S. aureus cells, which then multiply and amplify11 the phage signal. Following a two-hour incubation(孵化,潜伏) , the researchers break up proteins from the phage shell into component12 peptides using a trypsin digest technique.