酗酒会导致肌肉力量衰竭

Muscle weakness is a common symptom of both long-time alcoholics¹ and patients with mitochondrial（线粒体的） disease. Now researchers have found a common link: mitochondria that are unable to self-repair. The results will be published online April 21 in The Journal of Cell Biology. The link to self-repair provides researchers both a new way to diagnose mitochondrial disease, and a new drug target. Mitochondria -- organelles（细胞器） that produce the energy needed for muscle, brain, and every other cell in the body -- repair their broken components² by fusing with other mitochondria and exchanging their contents. Damaged parts are segregated³ for recycling and replaced with properly functioning proteins donated from healthy mitochondria.

 

While fusion⁴ is one major method for mitochondrial quality control in many types of cells, researchers have puzzled over the repair mechanism⁵ in skeletal muscle -- a type of tissue that relies constantly on mitochondria for power, making repair a frequent necessity. However, mitochondria are squeezed so tightly in between the packed fibers⁶ of muscle cells, that most researchers assumed that fusion among mitochondria in this tissue type was impossible.

 

An inkling that fusion might be important for the normal muscle function came from research on two mitochondrial diseases: Autosomal Dominant⁷ Optical Atropy (ADOA) disease, and a type of Charcot-Marie-Tooth disease (CMT). A symptom of both disease is muscle weakness and patients with both these diseases carry a mutation⁸ in one of the three genes⁹ involved in mitochondrial fusion.

 

To investigate whether mitochondria in the muscle could indeed fuse to regenerate¹¹, first author Veronica Eisner, Ph.D., a postdoctoral fellow at Thomas Jefferson University created a system to tag the mitochondria in skeletal muscle of rats with two different colors and then watch if they mingled¹². First, she created a rat model whose mitochondria expressed the color red at all times. She also genetically¹³ engineered the mitochondria in the cells to turn green when zapped with a laser, creating squares of green-shining mitochondria within the red background. To her surprise, the green mitochondria not only mingled with the red, exchanging contents, but were also able to travel to other areas where only red-colored mitochondria had been. The results were exciting in that they showed "for the first time that mitochondrial fusion occurs in muscle cells," says Dr. Eisner.

 

The researcher team, led by Dr. Gyorgy Hajnoczky, M.D., Ph.D., Director of Jefferson's MitoCare Center and professor in the department of Pathology, Anatomy¹⁴ & Cell Biology, then showed that of the mitofusin (Mfn) fusion proteins, Mfn1 was most important in skeletal muscle cells.

 

Once they had identified Mfn1, they were able to test whether mitochondrial fusion was the culprit in other examples of muscle weakness, such as alcoholism. One long-term symptom of alcoholism is the loss of muscle strength. The researchers showed that the Mfn1 abundance went down as much as 50 percent in rats on a regular alcohol diet-while other fusion proteins were unchanged, and that this decrease was coupled with a massive decrease in mitochondrial fusion. When Mfn1 was restored, so was the mitochondrial fusion. They also linked the decreased Mfn1 and mitochondrial fusion to increased muscle fatigue¹⁵.

 

"That alcohol can have a specific effect on this one gene¹⁰ involved in mitochondrial fusion suggests that other environmental factors may also specifically alter mitochondrial fusion and repair," says Dr. Hajnoczky.

 

"The work provides more evidence to support the concept that fission¹⁶ and fusion -- or mitochondrial dynamics¹⁷ -- may be responsible for more than just a subset of mitochondrial diseases we know of," says Dr. Hajnoczky. "In addition, knowing the proteins involved in the process gives us the possibility of developing a drug."

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