维生素B12能加速蠕虫生长

Everyday our cells take in nutrients² from food and convert them into the building blocks that make life possible. However, it has been challenging to pinpoint³ exactly how a single nutrient¹ or vitamin changes gene⁴ expression and physiology⁵. Scientists at the University of Massachusetts Medical School have developed a novel interspecies model system that allows these questions to be answered. In a study appearing in the journal Cell, UMMS researchers use this new approach to show how bacterially supplied vitamin B12 changes gene expression, development and fertility in the model organism C. elegans. "In mammals, micronutrients are provided by a combination of diet and gut⁶ flora⁷," said A.J. Marian Walhout, PhD, co-director of the Program in Systems Biology and professor of molecular⁸ medicine at UMMS and senior author of the study. "We've developed a powerful approach that can be used to unravel⁹（阐明） the complex interaction between nutrients, gene expression and physiology by systematically¹⁰ studying both the predator¹¹ (worm) and the prey¹² (bacteria). With it we can begin to answer important questions about how what we eat affects how we function."

 

The key to the study was a set of complimentary¹³（赠送的，称赞的） genetic¹⁴ screens performed on the transparent¹⁵ roundworm C. elegans and two kinds of bacteria that comprised the worm's diet -- Comamonas and E. coli. In a pair of papers published last year, Walhout and colleagues described dramatic changes in gene expression between worms fed only Comamonas and those fed only E. coli bacteria. Linked to these genetic changes were profound physiological¹⁶ differences between the worms. Comamonas-fed worms developed faster and were less fertile than their E. coli-fed counterparts.

 

By genetically¹⁷ dissecting¹⁸ the two bacteria and using a special C. elegans strain developed to sense changes to diet-related gene expression, Walhout and colleagues were able to zero in on a set of genes¹⁹ present in Comamonas but absent from E. coli. Further testing confirmed that these genes were responsible for producing vitamin B12 in Comamonas and it was the presence of the micronutrient that accounted for the genetic and physiological differences seen between the worms on different diets.

 

Importantly, Walhout found that vitamin B12 fulfills²⁰ two important functions in C. elegans: It helps regulate development through the methionine/SAM cycle, which is needed for the production of cell membranes²¹ in new cells. It also alleviates²² potentially toxic²³ buildups of the short-chain fatty acid propionic acid, which can alter gene expression or harm cells.

 

"C. elegans fed E. coli are actually vitamin B12 deficient²⁴ and this reflects only one natural state of the animal," said Walhout. "Because E. coli has been the standard laboratory diet for decades it would be interesting to study other characteristics of the worm, such as behavior, mating and movement, on a vitamin B12 rich diet."

 

Walhout and colleagues say that this system can also be adapted to identify genetic and physiological changes caused by other micronutrients in C. elegans. With the proper human analogs, it's possible that we could one day predict the precise interaction between diet, gene expression and physiology that occurs when we eat a carrot, hamburger, steak or any other food. Doing so might someday lead to new insights into a variety of conditions or diseases such as high cholesterol²⁵, heart disease, diabetes²⁶ and obesity²⁷. It can also be used to explore the precise benefits of bacteria found in gut flora.

 

"It turns out a single transgenic worm is a powerful tool for exploring the complex interaction between macro and micronutrients, gene expression and physiology," said Emma Watson, a doctoral student in the Walhout Lab and first author on the Cell study.

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