细胞核附近的DNA对新陈代谢有重要作用

New research from the University of California, Davis, shows that the tiny proportion of a cell's DNA¹ that is located outside the cell nucleus² has a disproportionately large effect on a cell's metabolism³. The work, with the model plant Arabidopsis, may have implications for future treatments for inherited diseases in humans. Plant and animal cells carry most of their genes⁴ on chromosomes⁵ in the nucleus, separated from the rest of the cell. However, they also contain a small number of genes in organelles that lie outside the nucleus. These are the mitochondria（线粒体）, which generate energy for animal and plant cells, and chloroplasts, which carry out photosynthesis⁶ in plant cells.

 

The influence of genes outside the nucleus was known to an earlier generation of field ecologists and crop breeders, said Dan Kliebenstein, professor in the UC Davis Department of Plant Sciences and Genome Center and senior author on the paper published Oct. 8 in the online journal eLife. This is the first time that the effect has been quantified with a genomic approach, he said.

 

Bindu Joseph, a postdoctoral researcher in Kliebenstein's lab, and Kliebenstein studied how variation in 25,000 nuclear genes and 200 organellar genes affected⁷ the levels of thousands of individual chemicals, or metabolites, in leaf tissue from 316 individual Arabidopsis plants.

 

They found that 80 percent of the metabolites measured were directly affected by variation in the organellar genes -- about the same proportion that were affected by variation among the much larger number of nuclear genes. There were also indirect effects, where organellar genes regulated the activity of nuclear genes that in turn affected metabolism.

 

"At first it's surprising, but at another level you almost expect it," Kliebenstein said. "These organelles produce energy and sugar for cells, so they are very important."

 

Similar effects could also occur in mammalian cells, Kliebenstein said. That has implications for in vitro fertilization therapies aimed at preventing diseases caused by faulty mitochondria being passed from mother to child. The British government recently proposed draft regulations for "three-parent embryos," created by taking a the nucleus from a fertilized⁸ egg and putting it into an egg cell from a third donor⁹ with its own set of mitochondria. The technique has so far only been tested in animals.

 

"From what we can see in plants, there might be an issue, but it needs testing," Kliebenstein said.

 

Large population surveys that aim to link conditions such as obesity¹⁰ to specific genes should also take more account of organellar genes, he said.

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